Bailey & Scott’s Diagnostic Microbiology 15th Edition by Patricia M. Tille, ISBN-13: 978-0323681056

Description

Bailey & Scott’s Diagnostic Microbiology 15th Edition by Patricia M. Tille, ISBN-13: 978-0323681056

[PDF eBook eTextbook]

• Publisher: ‎ Elsevier; 15th edition (March 11, 2021)
• Language: ‎ English
• 1184 pages
• ISBN-10: ‎ 0323681050
• ISBN-13: ‎ 978-0323681056

Perfect your lab skills with the essential text for diagnostic microbiology! Bailey & Scott’s Diagnostic Microbiology, 15th Edition is known as the #1 bench reference for practicing microbiologists and as the preeminent text for students in clinical laboratory science programs. With hundreds of full-color illustrations and step-by-step methods for procedures, this text provides a solid, basic understanding of diagnostic microbiology and also covers more advanced techniques such as matrix-assisted laser desorption time-of-flight mass spectrometry. Written by noted CLS educator Dr. Patricia Tille, Diagnostic Microbiology has everything you need to get accurate lab test results in class and in clinical practice.

Table of Contents:

Cover image

Title page

Disclaimer

Table of Contents

Copyright

Dedication

Reviewers

Contributors

Preface

Part I. Basic Medical Microbiology

1. Microbial Taxonomy

Classification

Nomenclature

Identification

2. Bacterial Genetics, Metabolism, and Structure

Bacterial Genetics

Bacterial Metabolism

Structure and Function of the Bacterial Cell

3. Host-Microorganism Interactions

The Encounter Between Host and Microorganism

Microorganism Colonization of Host Surfaces

Microorganism Entry, Invasion, and Dissemination

Outcome and Prevention of Infectious Diseases

General Principles in Clinical Microbiology

Section 1. Safety and Specimen Management

4. Laboratory Safety

Sterilization, Disinfection, and Decontamination

Chemical Safety

Fire Safety

Electrical Safety

Handling of Compressed Gases

Biosafety

Exposure Control Plan

Employee Education and Orientation

Disposal of Hazardous Waste

Standard Precautions

Laboratory Design and Engineering Controls

Biological Safety Levels

Mailing Biohazardous Materials

5. Specimen Management

General Concepts for Specimen Collection and Handling

Specimen Preservation

Specimen Work-Up

Expediting Results Reporting: Computerization

Section 2. Approaches to Diagnosis of Infectious Diseases

6. Role of Microscopy

Bright-Field (Light) Microscopy

Phase-Contrast Microscopy

Fluorescent Microscopy

Dark-Field Microscopy

Digital Automated Microscopy

Digital Holographic Microscopy

7. Overview of Conventional Cultivation and Systems for Identification

Organism Identification

Principles of Bacterial Cultivation

Bacterial Cultivation

Principles of Identification

Principles of Phenotypic Identification Schemes

Commercial Identification Systems and Automation

8. Nucleic Acid–Based Analytic Methods for Microbial Identification and Characterization

Overview of Nucleic Acid–Based Methods

Postamplification End-Point Analysis

9. Overview of Immunochemical Methods Used for Organism Detection

Features of the Immune Response

Features of the Humoral Immune Response Useful in Diagnostic Testing

Interpretation of Serologic Tests

Production of Antibodies for Use in Laboratory Testing

Immunoglobulin M Clinical Significance

Separating Immunoglobulin M from Immunoglobulin G for Serologic Testing

Principles of Immunochemical Methods Used for Organism Detection

Summary

Section 3. Evaluation of Antimicrobial Activity

10. Principles of Antimicrobial Action and Resistance

Antimicrobial Action

Mechanisms of Antibiotic Resistance

11. Laboratory Methods and Strategies for Antimicrobial Susceptibility Testing

Goal and Limitations

Testing Methods

Laboratory Strategies for Antimicrobial Susceptibility Testing

Accuracy

Communication

Bacteriology

Section 1. Principles of Identification

12. Overview of Bacterial Identification Methods and Strategies

Rationale for Approaching Organism Identification

Future Trends of Organism Identification

Section 2. Catalase-Positive, Gram-Positive Cocci

13. Staphylococcus, Micrococcus, and Similar Organisms

General Characteristics

Epidemiology

Pathogenesis and Spectrum of Disease

Laboratory Diagnosis

Antimicrobial Susceptibility Testing and Therapy

Prevention

Section 3. Catalase-Negative, Gram-Positive Cocci

14. Streptococcus, Enterococcus, and Similar Organisms

General Characteristics

Epidemiology

Pathogenesis and Spectrum of Disease

Laboratory Diagnosis

Antimicrobial Susceptibility Testing and Therapy

Prevention

Section 4. Non-Branching, Catalase-Positive, Gram-Positive Bacilli

15. Bacillus and Similar Organisms

General Characteristics

Laboratory Diagnosis

Antimicrobial Susceptibility Testing and Therapy

Prevention

16. Listeria, Corynebacterium, and Similar Organisms

General Characteristics

Epidemiology

Pathogenesis and Spectrum of Disease

Laboratory Diagnosis

Antimicrobial Susceptibility Testing and Therapy

Prevention

Treatment

Section 5. Non-Branching, Catalase-Negative, Gram-Positive Bacilli

17. Erysipelothrix, Lactobacillus, and Similar Organisms

General Characteristics

Epidemiology

Pathogenesis and Spectrum of Disease

Laboratory Diagnosis

Prevention

Section 6. Branching or Partially Acid-Fast, Gram-Positive Bacilli

18. Nocardia, Streptomyces, Rhodococcus, and Similar Organisms

General Characteristics

Epidemiology and Pathogenesis

Laboratory Diagnosis

Antimicrobial Susceptibility Testing and Therapy

Prevention

Section 7. Gram-Negative Bacilli and Coccobacilli (MacConkey-Positive, Oxidase-Negative)

19. Enterobacterales

General Characteristics

Epidemiology

Pathogenesis and Spectrum of Diseases

Specific Organisms

Laboratory Diagnosis

Prevention

20. Acinetobacter, Stenotrophomonas, and Other Organisms

General Characteristics

Epidemiology

Pathogenesis and Spectrum of Disease

Laboratory Diagnosis

Cultivation

Antimicrobial Resistance and Antimicrobial Susceptibility Testing

Antimicrobial Therapy

Prevention

Section 8. Gram-Negative Bacilli and Coccobacilli (MacConkey-Positive, Oxidase-Positive)

21. Pseudomonas, Burkholderia, and Similar Organisms

General Characteristics

Epidemiology

Pathogenesis and Spectrum of Disease

Laboratory Diagnosis

Antimicrobial Susceptibility Testing and Therapy

Prevention

22. Achromobacter, Rhizobium, Ochrobactrum, and Similar Organisms

General Characteristics

Epidemiology

Pathogenesis and Spectrum of Disease

Laboratory Diagnosis

Antimicrobial Susceptibility Testing and Therapy

Prevention

23. Chryseobacterium, Sphingobacterium, and Similar Organisms

General Characteristics

Epidemiology

Pathogenesis and Spectrum of Disease

Laboratory Diagnosis

Antimicrobial Susceptibility Testing and Therapy

Prevention

24. Alcaligenes, Comamonas, and Similar Organisms

General Characteristics

Epidemiology

Pathogenesis and Spectrum of Disease

Laboratory Diagnosis

Antimicrobial Susceptibility Testing and Therapy

Prevention

25. Vibrio, Aeromonas, Plesiomonas shigelloides, and Chromobacterium violaceum

General Characteristics

Epidemiology

Pathogenesis and Spectrum of Disease

Laboratory Diagnosis

Antimicrobial Susceptibility Testing and Therapy

Prevention

Section 9. Gram-Negative Bacilli and Coccobacilli (MacConkey-Negative, Oxidase-Positive)

26. Sphingomonas and Similar Organisms

General Considerations

Epidemiology, Spectrum of Disease, and Antimicrobial Therapy

Laboratory Diagnosis

Prevention

27. Moraxella and Neisseria spp.

General Characteristics

Epidemiology, Spectrum of Disease, and Antimicrobial Therapy

Laboratory Diagnosis

Antimicrobial Susceptibility

Prevention

28. Eikenella corrodens and Similar Organisms

General Characteristics

Epidemiology, Spectrum of Disease, and Antimicrobial Therapy

Laboratory Diagnosis

Serodiagnosis

Prevention

29. Pasteurella and Similar Organisms

General Characteristics and Taxonomy

Epidemiology, Spectrum of Disease, and Antimicrobial Therapy

Laboratory Diagnosis

Serodiagnosis

Serodiagnosis

Prevention

30. Actinobacillus, Kingella, Cardiobacterium, Capnocytophaga, and Similar Organisms

General Characteristics

Epidemiology, Pathogenesis, and Spectrum of Disease and Antimicrobial Therapy

Laboratory Diagnosis

Nucleic Acid Detection

Serodiagnosis

Prevention

Section 10. Gram-Negative Bacilli and Coccobacilli (MacConkey-Negative, Oxidase-Variable)

31. Haemophilus

General Characteristics

Epidemiology

Pathogenesis and Spectrum of Disease

Laboratory Diagnosis

Antimicrobial Susceptibility Testing and Therapy

Prevention

Section 11. Gram-Negative Bacilli That Are Optimally Recovered on Special Media

32. Bartonella

Bartonella

Direct Detection Methods

33. Campylobacter, Arcobacter, and Helicobacter

Campylobacter and Arcobacter

Helicobacter spp

34. Legionella

General Characteristics

Pathogenesis and Spectrum of Disease

Laboratory Diagnosis

Antimicrobial Susceptibility Testing and Therapy

Prevention

35. Brucella

General Characteristics

Epidemiology and Pathogenesis

Spectrum of Disease

Laboratory Diagnosis

Antimicrobial Susceptibility Testing and Therapy

Prevention

36. Bordetella pertussis, Bordetella parapertussis, and Related Species

General Characteristics

Spectrum of Disease

Laboratory Diagnosis

Antimicrobial Susceptibility Testing and Therapy

Prevention

37. Francisella

General Characteristics

Epidemiology and Pathogenesis

Spectrum of Disease

Laboratory Diagnosis

Antimicrobial Susceptibility Testing and Therapy

Prevention

38. Streptobacillus spp. and Spirillum minus

Streptobacillus spp

Spirillum minus

Section 12. Gram-Negative Cocci

39. Neisseria and Moraxella catarrhalis

General Characteristics

Epidemiology

Pathogenesis and Spectrum of Disease

Laboratory Diagnosis

Antimicrobial Susceptibility Testing and Therapy

Prevention

Section 13. Anaerobic Bacteriology

40. Overview and General Laboratory Considerations

General Characteristics

Specimen Collection and Transport

Macroscopic Examination of Specimens

Direct Detection Methods

Specimen Processing

Anaerobic Media

Approach to Identification

Antimicrobial Susceptibility Testing and Therapy

41. Overview of Anaerobic Organisms

Epidemiology

Pathogenesis and Spectrum of Disease

Nucleic Acid Detection and MALDI-TOF MS (Gram-Positive)

Nucleic Acid Detection and MALDI-TOF MS (Gram-Negative)

Nucleic Acid Detection and MALDI-TOF MS

Prevention

Section 14. Mycobacteria and Other Bacteria With Unusual Growth Requirements

42. Mycobacteria

Mycobacterium tuberculosis Complex

Nontuberculous Mycobacteria

Laboratory Diagnosis of Mycobacterial Infections

Antimicrobial Susceptibility Testing and Therapy

Prevention

43. Obligate Intracellular and Nonculturable Bacterial Agents

Chlamydia

Rickettsia, Orientia, Anaplasma, and Ehrlichia

Coxiella

Tropheryma whipplei

Klebsiella granulomatis

44. Cell Wall–Deficient Bacteria: Mycoplasma and Ureaplasma

General Characteristics

Epidemiology and Pathogenesis

Spectrum of Disease

Laboratory Diagnosis

Cultivation

Susceptibility Testing and Therapy

Prevention

45. The Spirochetes

Treponema

Borrelia

Brachyspira

Leptospira

Prevention

Part IV. Parasitology

46. Overview of the Methods and Strategies in Parasitology

Epidemiology

Pathogenesis and Spectrum of Disease

Laboratory Diagnosis

Approach to Identification

Prevention

Ectoparasites

47. Intestinal Protozoa

Amoebae

Flagellates

Ciliates

Sporozoa (Apicomplexa)

Microsporidia

48. Blood and Tissue Protozoa

Plasmodium spp

Babesia spp

Trypanosoma spp

Leishmania spp

49. Protozoa From Other Body Sites

Free-Living Amoebae

Naegleria fowleri

Acanthamoeba spp

Acanthamoeba Keratitis

Balamuthia mandrillaris

Trichomonas vaginalis

Toxoplasma gondii

50. Intestinal Nematodes

Ascaris lumbricoides

Enterobius vermicularis

Strongyloides stercoralis

Trichostrongylus spp

Trichuris trichiura

Capillaria philippinensis

Hookworms

Ancylostoma duodenale

Necator americanus

Ancylostoma ceylonicum, Ancylostoma braziliense, and Ancylostoma caninum

Results and Reporting

51. Tissue Nematodes

Trichinella spp

Toxocara canis (Visceral Larva Migrans) and Toxocara cati (Ocular Larva Migrans)

Baylisascaris procyonis (Neural Larva Migrans)

Ancylostoma braziliense and Ancylostoma caninum (Cutaneous Larva Migrans)

Dracunculus medinensis

Parastrongylus cantonensis (Cerebral Angiostrongyliasis)

Parastrongylus costaricensis (Abdominal Angiostrongyliasis)

Gnathostoma spinigerum

Capillaria hepatica

Dirofilaria immitis and Other Species

52. Blood and Tissue Filarial Nematodes

Wuchereria bancrofti

Brugia malayi and Brugia timori

Loa loa

Onchocerca volvulus

Mansonella spp. (M. ozzardi, M. streptocerca, M. perstans)

Dirofilaria spp. (D. immitis, D. repens, D. tenuis)

53. Intestinal Cestodes

Diphyllobothrium latum

Dipylidium caninum

Hymenolepis nana

Hymenolepis diminuta

Taenia solium

Taenia saginata

Taenia asiatica

Taenia crassiceps

54. Tissue Cestodes

Taenia solium

Echinococcus granulosus Complex

Echinococcus multilocularis

Echinococcus oligarthrus and Echinococcus vogeli

Taenia multiceps and Other Species

Taenia serialis

Spirometra mansonoides

55. Intestinal Trematodes

Echinostoma spp

Fasciolopsis buski General Characteristics

Gastrodiscoides hominis

Heterophyes: Metagonimus yokogawai, Centrocestus spp., Haplorchis spp., Stellantchamus spp., and Pygidiopsis spp. General Characteristics

56. Liver and Lung Trematodes

The Liver Flukes

The Lung Flukes

57. Blood Trematodes

General Characteristics

Epidemiology

Pathology and Spectrum of Disease

Laboratory Diagnosis

Therapy

Prevention

Part V. Mycology

58. Overview of Fungal Identification Methods and Strategies

Epidemiology

General Features of the Fungi

Taxonomy of the Fungi

Clinical Classification of the Fungi

Pathogenesis and Spectrum of Disease

Laboratory Diagnosis

General Considerations for the Identification of Yeasts

General Considerations for the Identification of Molds

General Morphologic Features of the Molds

Clinical Relevance for Fungal Identification

Laboratory Safety

Prevention

59. Hyaline Molds, Mucorales, Basidiobolales, Entomophthorales, Dermatophytes, and Opportunistic and Systemic Mycoses

The Mucorales

The Entomophthorales and Basidiobolales

The Dermatophytes

The Opportunistic Mycoses

Systemic Mycoses

60. Dematiaceous (Melanized) Molds

General Characteristics

Epidemiology and Pathogenesis Superficial Infections

Pathogenesis and Spectrum of Disease

Laboratory Diagnosis

61. Atypical and Parafungal Agents

Pneumocystis

Epidemiology

Pathogenesis and Spectrum of Disease

Laboratory Diagnosis

Rare Atypical and Parafungal Agents

62. The Yeasts and Yeastlike Organisms

General Characteristics

Epidemiology

Pathogenesis and Spectrum of Disease

Laboratory Diagnosis

Commercial Yeast Identification Systems

63. Antifungal Susceptibility Testing, Therapy, and Prevention

Antifungal Susceptibility Testing

Antifungal Therapy and Prevention

Part VI. Virology

64. Overview of the Methods and Strategies in Virology

General Characteristics

Epidemiology

Pathogenesis and Spectrum of Disease

Prevention and Therapy

Viruses That Cause Human Diseases

Laboratory Diagnosis

65. Viruses in Human Disease

Viruses in Human Disease

Adenoviridae

Arenaviridae

Astroviridae

Caliciviridae

Coronaviridae

Filoviridae

Flaviviridae

Hantaviridae

Hepadnaviridae

Hepeviridae

Herpesviridae

Herpesviruses

Varicella-Zoster Virus

Epstein-Barr Virus

Cytomegalovirus

Orthomyxoviridae

Papillomaviruses

Paramyxoviridae

Measles Virus

Parvoviridae

Picornaviridae

Enteroviruses, Parechoviruses, and Polioviruses

Rhinovirus

Hepatitis A Virus

Pneumoviridae

Polyomaviridae

Poxviridae

Reoviridae

Retroviridae

Rhabdoviridae

Togaviridae

Prions in Human Disease

66. Antiviral Therapy, Susceptibility Testing, and Prevention

Antiviral Therapy

Antiviral Resistance

Methods of Antiviral Susceptibility Testing

Prevention of Other Viral Infections

Part VII. Diagnosis by Organ System

67. Bloodstream Infections

General Considerations

Detection of Bacteremia

Special Considerations for Other Relevant Organisms Isolated From Blood

68. Infections of the Lower Respiratory Tract

General Considerations

Diseases of the Lower Respiratory Tract

Laboratory Diagnosis of Lower Respiratory Tract Infections

69. Upper Respiratory Tract Infections and Other Infections of the Oral Cavity and Neck

General Considerations

Diseases of the Upper Respiratory Tract, Oral Cavity, and Neck

Diagnosis of Upper Respiratory Tract Infections

Diagnosis of Infections in the Oral Cavity and Neck

70. Meningitis and Other Infections of the Central Nervous System

General Considerations

Shunt Infections

Laboratory Diagnosis of Central Nervous System Infections

71. Infections of the Eyes, Ears, and Sinuses

Eyes

Ears

Sinuses

72. Infections of the Urinary Tract

General Considerations

Infections of the Urinary Tract

Laboratory Diagnosis of Urinary Tract Infections

73. Genital Tract Infections

General Considerations

Genital Tract Infections

Laboratory Diagnosis of Genital Tract Infections

74. Gastrointestinal Tract Infections

Anatomy

Resident Gastrointestinal Microbiome

Gastroenteritis

Other Infections of the Gastrointestinal Tract

Laboratory Diagnosis of Gastrointestinal Tract Infections

75. Skin, Soft Tissue, and Wound Infections

General Considerations

Skin and Soft Tissue Infections

Laboratory Diagnostic Procedures

76. Normally Sterile Body Fluids, Bone and Bone Marrow, and Solid Tissues

Specimens From Sterile Body Sites

Laboratory Diagnostic Procedures

Part VIII. Clinical Laboratory Management

77. Quality in the Clinical Microbiology Laboratory

Quality Program

Specimen Collection and Transport

Standard Operating Procedure Manual

Personnel

Reference Laboratories

Patient Reports

Proficiency Testing

Performance Checks

Antimicrobial Susceptibility Tests

Maintenance of Quality Control Records

Maintenance of Reference Quality Control Stocks

Quality Assurance Program

Types of Quality Assurance Audits

Conducting a Quality Assurance Audit

Continuous Daily Monitoring

78. Infection Control

Incidence of Health Care–Associated Infections

Types of Health Care–Associated Infections

Emergence of Antibiotic-Resistant Microorganisms

Hospital Infection Control Programs

Role of the Microbiology Laboratory

Characterizing Strains Involved in an Outbreak

Preventing Health Care–Associated Infections

Surveillance Methods

79. Sentinel Laboratory Response to Bioterrorism

General Considerations

Government Laws and Regulations

Laboratory Response Network

Index

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